陈忠课题组《Brain structure and function》最新研究成果

2013-06-26 超级管理员  浏览量 :   590

2013年6月,卫生部医学神经生物学重点实验室和浙江省神经生物学重点实验室陈忠教授课题组在国际神经科学著名杂志《Brain structure and function》(IF=7.8)上发表了题为《Low-frequency stimulation inhibits epileptogenesis by modulating the early network of the limbic system as evaluated in amygdala kindling model》的研究文章。文章报道了低频率电刺激技术作用于癫痫灶点处抑制癫痫的早期的发展进程,并解析了癫痫早期大脑网络改变的病理特征。

低频率电刺激技术是近年来兴起的治疗难治性癫痫的新技术,具有可调节性,可逆性,副作用小等优点。课题组研究发现低频率电刺激不仅能有效的抑制癫痫的发作,对癫痫的形成也有明显抑制作用。在该研究中课题组综合运用行为学分析,在体电生理技术和小动物断层扫描等多种手段研究了低频率电刺激癫痫灶点抑制癫痫早期发病进程,发现了癫痫早期发病时伴随癫痫灶点周围大脑边缘系统脑区能量特异性降低。这一结果不仅有助于人们进一步认识低频率电刺激的作用特点,同时也为解析癫痫的形成机制提供了参考,对临床预测癫痫的早期发病具有重要指导意义。

研究癫痫的发病机制和治疗手段是该研究组的重要研究方向之一,文章的共同第一作者是博士生汪仪和博士后许正浩。课题受国家自然科学基金重点项目和浙江省自然科学基金重点项目等资助。

原文链接http://www.ncbi.nlm.nih.gov/pubmed/23754239

A research group headed by Professor CHEN Zhong at Department of Pharmacology, Key Laboratory of Medical Neurobiology of the Ministry of Health of China and Zhejiang Province Key Laboratory of Neurobiology, College of pharmaceutical sciences, Zhejiang University has recently published a research paper titledLow-frequency stimulation inhibits epileptogenesis by modulating the early network of the limbic system as evaluated in amygdala kindling model in the internationally renowned neuroscience journal Brain structure and function in June 2013. The article reported thatmodulation of the early network in the limbic system is crucial for the anti-epileptogenic effect of LFS in amygdaloid-kindling rats.

Low-frequency stimulation (LFS) is emerging as a new and promising option for the treatment of intractable epilepsy with the advantages of reversibility and adjustability and is relatively safer than high-frequency stimulation. However, its mechanisms of action remain unclear. In their study, they have provided the first evidence that the early stages are crucial for LFS inhibiting epileptogenesis by modulating the early network of limbic system. The hypometabolism in the limbic network may be an important biomarker for the beginning of epileptogenesis in temporal lobe epilepsy. So far, human clinical trials attempting to prevent epilepsy have been few and universally unsuccessful; one possible reason may be that the treatment was not administered within the critical period. Therefore, the hypometabolism in the limbic network may be an important biomarker for the beginning of epileptogenesis in temporal lobe epilepsy and this presents the possibility of early intervention and predictive potential in the future management of epilepsy patients.

Studying the mechanism of epileptogenesis and treatment is one of the important research directions for Professor Chen’s research group. Co-first authors of the article are Dr. Yi Wang and Dr. Zheng-hao Xu. The study was funded by the Key Project of National Natural Science Foundation of China and the Key Project of Zhejiang Provincial Natural Science Foundation.

Wang Y, Xu Z, Cheng H, Guo Y, Xu C, Wang S, Zhang J, Ding M, Chen Z.Low-frequency stimulation inhibits epileptogenesis by modulating the early network of the limbic system as evaluated in amygdala kindling model.Brain Struct Funct. 2013 Jun 11.

Source

Key Laboratory of Medical Neurobiology of the Ministry of Health of China, Department of Pharmacology, Zhejiang Province Key Laboratory of Neurobiology, School of Medicine, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058, China.

 

Abstract

Low-frequency stimulation (LFS) is emerging as a new option for the treatment of epilepsy. The present study was designed to determine whether there is a crucial period for the treatment of epileptogenesis with LFS. LFS was delivered at different time-points to evaluate its anti-epileptogenic effect on amygdala-kindling rats. 18F-fluorodeoxyglucose small-animal positron-emission tomography (microPET) and multi-channel EEG recording (MER) were used to investigate the dynamics of brain networks during epileptogenesis and LFS treatment. Interestingly, LFS delivered in the first 7 days significantly retarded the progression of behavioral seizure stages and shortened the afterdischarge duration (ADD), LFS delivered throughout the whole process resulted in similar effects. However, if LFS was delivered at the beginning of seizure stage 2 or 3 (5 ± 0.3 days during kindling acquisition), it had no anti-epileptogenic effect and even prolonged the ADD and enhanced synchronization of the EEGs. MicroPET study revealed a notable hypometabolism in the amygdala, piriform cortex, entorhinal cortex and other regions in the limbic system during the period from seizure stage 0 to stage 2 or 3. The glucose metabolism in those regions was specifically increased by LFS. MER further verified that an early network of afterdischarge spread was formed in those brain regions during kindling acquisition. Thus, we provided direct evidence that modulation of the early network in the limbic system is crucial for the anti-epileptogenic effect of LFS in amygdaloid-kindling rats.

PMID: 23754239 [PubMed - as supplied by publisher]

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